Akiyama, Benjamin M Gomez, Anastassia Stone, Michael D
Published in
The Journal of biological chemistry
The ends of linear chromosomes are extended by telomerase, a ribonucleoprotein complex minimally consisting of a protein subunit called telomerase reverse transcriptase (TERT) and the telomerase RNA (TER). TERT functions by reverse transcribing a short template region of TER into telomeric DNA. Proper assembly of TERT and TER is essential for telom...
Mukherjee, Sohini Partch, Carrie L Lehotzky, Rebecca E Whitham, Cecilia V Chu, Hiutung Bevins, Charles L Gardner, Kevin H Hooper, Lora V
Published in
The Journal of biological chemistry
Members of the RegIII family of intestinal C-type lectins are directly antibacterial proteins that play a vital role in maintaining host-bacterial homeostasis in the mammalian gut, yet little is known about the mechanisms that regulate their biological activity. Here we show that the antibacterial activities of mouse RegIIIgamma and its human ortho...
Parnell, Emily J Yu, Yaxin Lucena, Rafael Yoon, Youngdae Bai, Lu Kellogg, Douglas R Stillman, David J
Published in
The Journal of biological chemistry
The RTS1 gene encodes a subunit of the PP2A phosphatase that regulates cell cycle progression. Ace2 and Swi5 are cell cycle-regulated transcription factors, and we recently showed that phosphorylation of Ace2 and Swi5 is altered in an rts1 mutant. Here we examine expression of Ace2 and Swi5 target genes and find that an rts1 mutation markedly reduc...
Saddic, Louis A West, Lisandra E Aslanian, Aaron Yates, John R 3rd Rubin, Seth M Gozani, Or Sage, Julien
Published in
The Journal of biological chemistry
The retinoblastoma tumor suppressor (RB) is a central cell cycle regulator and tumor suppressor. RB cellular functions are known to be regulated by a diversity of post-translational modifications such as phosphorylation and acetylation, raising the possibility that RB may also be methylated in cells. Here we demonstrate that RB can be methylated by...
Burke, Jason R Deshong, Alison J Pelton, Jeffrey G Rubin, Seth M
Published in
The Journal of biological chemistry
Inactivation of the retinoblastoma protein (Rb) through phosphorylation is an important step in promoting cell cycle progression, and hyperphosphorylated Rb is commonly found in tumors. Rb phosphorylation prevents its association with the E2F transcription factor; however, the molecular basis for complex inhibition has not been established. We iden...
Liang, Fubo Luo, Yong Dong, Yuanshu Walls, Chad D Liang, Jiao Jiang, Hao-Yuan Sanford, Jeremy R Wek, Ronald C Zhang, Zhong-Yin
Published in
The Journal of biological chemistry
Phosphatase of regenerating liver 3 (PRL3) is up-regulated in cancer metastases. However, little is known of PRL3-mediated cellular signaling pathways. We previously reported that elevated PRL3 expression increases Src kinase activity, which likely contributes to the increased tumorigenesis and metastasis potential of PRL3. PRL3-induced Src activat...
Partch, Carrie L Card, Paul B Amezcua, Carlos A Gardner, Kevin H
Published in
The Journal of biological chemistry
The aryl hydrocarbon receptor nuclear translocator (ARNT) serves as the obligate heterodimeric partner for bHLH-PAS proteins involved in sensing and coordinating transcriptional responses to xenobiotics, hypoxia, and developmental pathways. Although its C-terminal transactivation domain is dispensable for transcriptional activation in vivo, ARNT ha...
