Carrie Partch Shields, Kf Thompson, Cl Selby, Cp Sancar, A
Published in
Proceedings of the National Academy of Sciences
The molecular oscillator that drives circadian rhythmicity in mammals obtains its near 24-h periodicity from posttranslational regulation of clock proteins. Activity of the major clock kinase casein kinase I (CKI) epsilon is regulated by inhibitory autophosphorylation. Here we show that protein phosphatase (PP) 5 regulates the kinase activity of CK...
Ma, Jian Ratan, Aakrosh Raney, Brian J Suh, Bernard B Miller, Webb Haussler, David
Published in
Proceedings of the National Academy of Sciences of the United States of America
We formalize the problem of recovering the evolutionary history of a set of genomes that are related to an unseen common ancestor genome by operations of speciation, deletion, insertion, duplication, and rearrangement of segments of bases. The problem is examined in the limit as the number of bases in each genome goes to infinity. In this limit, th...
Smoum, Reem Bar, Arik Tan, Bo Milman, Garry Attar-Namdar, Malka Ofek, Orr Stuart, Jordyn M Bajayo, Alon Tam, Joseph Kram, Vardit
...
Published in
Proceedings of the National Academy of Sciences of the United States of America
Bone mass is determined by a continuous remodeling process, whereby the mineralized matrix is being removed by osteoclasts and subsequently replaced with newly formed bone tissue produced by osteoblasts. Here we report the presence of endogenous amides of long-chain fatty acids with amino acids or with ethanolamine (N-acyl amides) in mouse bone. Of...
Huang, Y Baxter, R Smith, Bs Carrie Partch Colbert, Cl Deisenhofer, J
Published in
Proceedings of the National Academy of Sciences
Cryptochromes use near-UV/blue light to regulate a variety of growth and adaptive process. Recent biochemical studies demonstrate that the Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human (Cry-DASH) subfamily of cryptochromes have photolyase activity exclusively for single-stranded cyclobutane pyrimidine dimer (CPD)-containing DNA substra...
Gravel, Simon Henn, Brenna M Gutenkunst, Ryan N Indap, Amit R Marth, Gabor T Clark, Andrew G Yu, Fuli Gibbs, Richard A Bustamante, Carlos D
Published in
Proceedings of the National Academy of Sciences of the United States of America
High-throughput sequencing technology enables population-level surveys of human genomic variation. Here, we examine the joint allele frequency distributions across continental human populations and present an approach for combining complementary aspects of whole-genome, low-coverage data and targeted high-coverage data. We apply this approach to da...
Marlow, R Binnewies, M Sorensen, Lk Monica, Sd Strickland, P Forsberg, Ec Li, Dy Lindsay Hinck
Published in
Proceedings of the National Academy of Sciences
Formation of the vascular system within organs requires the balanced action of numerous positive and negative factors secreted by stromal and epithelial cells. Here, we used a genetic approach to determine the role of SLITs in regulating the growth and organization of blood vessels in the mammary gland. We demonstrate that vascularization of the gl...
Michael Stone Bryant, Z Crisona, Nj Smith, Sb Vologodskii, A Bustamante, C Cozzarelli, Nr
Published in
Proceedings of the National Academy of Sciences
Escherichia coli topoisomerase (Topo) IV is an essential type II Topo that removes DNA entanglements created during DNA replication. Topo IV relaxes (+) supercoils much faster than (-) supercoils, promoting replication while sparing the essential (-) supercoils. Here, we investigate the mechanism underlying this chiral preference. Using DNA binding...
Jeremy Sanford Bruzik, Jp
Published in
Proceedings of the National Academy of Sciences
Ser-Arg-rich (SR) proteins play numerous roles in spliceosome assembly and the regulation of splice-site selection. Whereas considerable attention has focused on the mechanistic details of SR protein activities, little is known concerning how these splicing regulators are controlled by the cell. Here we examined the subcellular localization of prec...
Militi, Stefania Maywood, Elizabeth S Sandate, Colby R Chesham, Johanna E Barnard, Alun R Parsons, Michael J Vibert, Jennifer L Joynson, Greg M Partch, Carrie L Hastings, Michael H
...
Published in
Proceedings of the National Academy of Sciences of the United States of America
The suprachiasmatic nucleus (SCN) defines 24 h of time via a transcriptional/posttranslational feedback loop in which transactivation of Per (period) and Cry (cryptochrome) genes by BMAL1-CLOCK complexes is suppressed by PER-CRY complexes. The molecular/structural basis of how circadian protein complexes function is poorly understood. We describe a...
Pradip Mascharak Smith, Mc Armstrong, Wh Burgess, Bk Holm, Rh
Published in
Proceedings of the National Academy of Sciences
Several properties of the FeMo-cofactor (co) of nitrogenase in N-methylformamide solution at ambient temperature have been investigated by means of 19F NMR spectroscopy. With C6H5CF3 reference signals the magnetic moment per Mo atom was found to be approximately equal to 3.9 BM, consistent with S = 3/2 ground state identified by other spectroscopic...
