The UCSC Ebola Genome Portal.
...Published in PLoS Currents
The meningitis scare in perspective.
Published in The Practitioner
Conserved role of intragenic DNA methylation in regulating alternative promoters.
...Published in Nature
Although it is known that the methylation of DNA in 5 promoters suppresses gene expression, the role of DNA methylation in gene bodies is unclear. In mammals, tissue- and cell type-specific methylation is present in a small percentage of 5 CpG island (CGI) promoters, whereas a far greater proportion occurs across gene bodies, coinciding with high...
Conversion of azomethine moiety to carboxamido group at cobalt(III) center in model complexes of Co-containing nitrile h...
Published in Inorganic Chemistry
The Co(III) complex of the Schiff base ligand N-2-mercaptophenyl-2 -pyridylmethyl-enimine (PyASH), namely, [Co(PyAS)(2)]Cl (1), has been synthesized via an improved method and its structure has been determined by X-ray crystallography. The two deprotonated ligands are arranged in mer configuration around the Co(III) center and the overall coordinat...
Near-infrared light activated release of nitric oxide from designed photoactive manganese nitrosyls: strategy, design, a...
Published in Journal of the American Chemical Society
Two new manganese complexes derived from the pentadentate ligand N,N-bis(2-pyridylmethyl)amine-N-ethyl-2-quinoline-2-carboxamide, PaPy2QH, where H is dissociable proton), namely, [Mn(PaPy2Q)(NO)]ClO4 (2) and [Mn(PaPy2Q)(OH)]ClO4 (3), have been synthesized and structurally characterized. The Mn(III) complex [Mn(PaPy2Q)(OH)]ClO4 (3), though insensiti...
Key stages in mammary gland development: the mammary end bud as a motile organ.
Published in Breast Cancer Research
In the rodent, epithelial end buds define the tips of elongating mammary ducts. These highly motile structures undergo repeated dichotomous branching as they aggressively advance through fatty stroma and, turning to avoid other ducts, they finally cease growth leaving behind the open, tree-like framework on which secretory alveoli develop during pr...
Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.
Published in The Journal of Cell Biology
During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain su...
Differential expression of novel potential regulators in hematopoietic stem cells.
Published in PLoS Genetics
The hematopoietic system is an invaluable model both for understanding basic developmental biology and for developing clinically relevant cell therapies. Using highly purified cells and rigorous microarray analysis we have compared the expression pattern of three of the most primitive hematopoietic subpopulations in adult mouse bone marrow: long-te...
Computational reconstruction of ancestral DNA sequences.
Published in Methods in Molecular Biology
This chapter introduces the problem of ancestral sequence reconstruction: given a set of extant orthologous DNA genomic sequences (or even whole-genomes), together with a phylogenetic tree relating these sequences, predict the DNA sequence of all ancestral species in the tree. Blanchette et al. (1) have shown that for certain sets of species (in pa...
Short class I major histocompatibility complex cytoplasmic tails differing in charge detect arbiters of lateral diffusio...
Published in Biophysical Journal
Directed and Brownian movement of class I major histocompatibility complex (MHC) molecules on cell membranes is implicated in antigen presentation. Previous studies indicated that the class I MHC cytoplasmic tail imposes constraints on the molecule s diffusion. Here we used single particle tracking to study the mobility of the wild-type mouse H-2L(...
