Hematopoietic stem cell-specific GFP-expressing transgenic mice generated by genetic excision of a pan-hematopoietic rep...

Perez-Cunningham, Jessica Boyer, Scott W Landon, Mark Forsberg, E Camilla

Published in Experimental hematology

Selective labeling of specific cell types by expression of green fluorescent protein (GFP) within the hematopoietic system would have great utility in identifying, localizing, and tracking different cell populations in flow cytometry, microscopy, lineage tracing, and transplantation assays. In this report, we describe the generation and characteriz...

Phosphono Bisbenzguanidines as Irreversible Dipeptidomimetic Inhibitors and Activity-Based Probes of Matriptase-2.

Häußler, Daniela Mangold, Martin Furtmann, Norbert Braune, Annett Blaut, Michael Bajorath, Jürgen Stirnberg, Marit Gütschow, Michael

Published in Chemistry (Weinheim an der Bergstrasse, Germany)

Matriptase-2, a type II transmembrane serine protease, plays a key role in human iron homeostasis. Inhibition of matriptase-2 is considered as an attractive strategy for the treatment of iron-overload diseases, such as hemochromatosis and β-thalassemia. In the present study, synthetic routes to nine dipeptidomimetic inactivators were developed. Fiv...

IGF2BP3 Modulates the Interaction of Invasion-Associated Transcripts with RISC.

Ennajdaoui, Hanane Howard, Jonathan M Sterne-Weiler, Timothy Jahanbani, Fereshteh Coyne, Doyle J Uren, Philip J Dargyte, Marija Katzman, Sol Draper, Jolene M Wallace, Andrew ...

Published in Cell reports

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) expression correlates with malignancy, but its role(s) in pathogenesis remains enigmatic. We interrogated the IGF2BP3-RNA interaction network in pancreatic ductal adenocarcinoma (PDAC) cells. Using a combination of genome-wide approaches, we have identified 164 direct mRNA targets of IGF...

Sedentary lifestyle in middle-aged women is associated with severe menopausal symptoms and obesity.

Blümel, Juan E Fica, Juan Chedraui, Peter Mezones-Holguín, Edward Zuñiga, María C Witis, Silvina Vallejo, María S Tserotas, Konstantinos Sánchez, Hugo Onatra, William ...

Published in Menopause (New York, N.Y.)

There was a high prevalence of sedentary lifestyle in this middle-aged Latin American female sample which was associated with more severe menopausal symptoms and obesity.

UCSC Data Integrator and Variant Annotation Integrator.

Hinrichs, Angie S Raney, Brian J Speir, Matthew L Rhead, Brooke Casper, Jonathan Karolchik, Donna Kuhn, Robert M Rosenbloom, Kate R Zweig, Ann S Haussler, David ...

Published in Bioinformatics (Oxford, England)

Supplementary data are available at Bioinformatics online.

Canonical, stable, general mapping using context schemes.

Novak, Adam M Rosen, Yohei Haussler, David Paten, Benedict

Published in Bioinformatics (Oxford, England)

Supplementary data are available at Bioinformatics online.

Progressive Chromatin Condensation and H3K9 Methylation Regulate the Differentiation of Embryonic and Hematopoietic Stem...

Ugarte, Fernando Sousae, Rebekah Cinquin, Bertrand Martin, Eric W Krietsch, Jana Sanchez, Gabriela Inman, Margaux Tsang, Herman Warr, Matthew Passegué, Emmanuelle ...

Published in Stem cell reports

Epigenetic regulation serves as the basis for stem cell differentiation into distinct cell types, but it is unclear how global epigenetic changes are regulated during this process. Here, we tested the hypothesis that global chromatin organization affects the lineage potential of stem cells and that manipulation of chromatin dynamics influences stem...

Mammary Stem Cell Self-Renewal Is Regulated by Slit2/Robo1 Signaling through SNAI1 and mINSC.

Ballard, Mimmi S Zhu, Anna Iwai, Naomi Stensrud, Michael Mapps, Aurelia Postiglione, Maira Pia Knoblich, Juergen A Hinck, Lindsay

Published in Cell reports

Tissue homeostasis requires somatic stem cell maintenance; however, mechanisms regulating this process during organogenesis are not well understood. Here, we identify asymmetrically renewing basal and luminal stem cells in the mammary end bud. We demonstrate that SLIT2/ROBO1 signaling regulates the choice between self-renewing asymmetric cell divis...

Design and construction of a silver(I)-loaded cellulose-based wound dressing: trackable and sustained release of silver ...

deBoer, T R Chakraborty, I Mascharak, P K

Published in Journal of materials science. Materials in medicine

Although application of silver nitrate and silver sulfadiazine have been shown to be effective in thwarting infections at burn sites, optimization of the delivery of bioactive silver (Ag(+)) remains as an obstacle due to rapid precipitation and/or insolubility of the silver sources. To circumvent these shortcomings, we have designed a silver(I) com...

Helicobacter pylori CheZ(HP) and ChePep form a novel chemotaxis-regulatory complex distinct from the core chemotaxis sig...

Lertsethtakarn, Paphavee Howitt, Michael R Castellon, Juan Amieva, Manuel R Ottemann, Karen M

Published in Molecular microbiology

Chemotaxis is important for Helicobacter pylori to colonize the stomach. Like other bacteria, H. pylori uses chemoreceptors and conserved chemotaxis proteins to phosphorylate the flagellar rotational response regulator, CheY, and modulate the flagellar rotational direction. Phosphorylated CheY is returned to its non-phosphorylated state by phosphat...